nkp46 pe cy7  (Miltenyi Biotec)

Journal: HemaSphere

Article Title: Interferon gamma‐mediated prevention of tumor progression in a mouse model of multiple myeloma

doi: 10.1002/hem3.70047

Figure Lengend Snippet: Single‐cell RNAseq of NK cells in C57Bl/6 and KaLwRij mice after transfer of 5TGM1 cells . (A) Representative fluorescent single‐color and merged images of C57Bl/6 (top) and KaLwRij (bottom) BM. Green, GFP/5TGM1; red, NK (NKp46 + ); blue, T (CD3 + ). Arrows indicate NK cells and T cells in respective images. ×200. Scale bar = 50 μm. (B) UMAP of NK cells from C57Bl/6 and KaLwRij mice. Two animals per strain at steady state and two animals per strain at d21 after 5TGM1. Colors indicate clusters obtained at resolution 0.4. (C) Dot plot depicting average expression levels of canonical genes used to identify clusters. (D) Split UMAP of NK cells per condition. (E) Bar graph depicting cluster distribution in individual mice. Colors indicate clusters. (F) Permutation plots showing changes of NK cluster size at Day 21 relative to steady state in C57Bl/6 (left) and KaLwRij (right) ( absLog2FD > 0.58; FDR < 0.05 was considered significant) . (G) Bar graphs showing frequencies of grouped mature clusters under steady state condition and Day 21 after tumor transfer. (H) Feature plots split per condition of genes associated with NK cell cytotoxicity; circled areas refer to indicated clusters colored using respective UMAP colors. (I) Feature plots split per condition of cytokines associated with NK activity; circled areas refer to indicated clusters colored using respective UMAP colors. (J) Feature plot split per condition of Cd69 activation marker; circled areas refer to indicated clusters colored using respective UMAP colors.

Article Snippet: The following antibodies were used for flow cytometry: CD3e‐PerCpCy5.5 (145‐2C11; eBioscience), CD3e‐PE/Dazzle594 (145‐2C11; Biolegend), CD8‐FITC (BD, 53‐6.7), CD11b‐APCeFluor780 (M1/70; eBioscience), CD19‐AF700 (eBio1D3; eBioscience), CD27‐BV510 (LG.3A10; Biolegend), CD31‐PE/CF594 (MEC13.3; BD), CD44‐BV510 (IM7; Biolegend), CD45‐APC/Cy7 (30‐F11; eBioscience), CD51‐PE (RMY‐7; eBioscience), CD69‐AF647 (H1.2F3; Biolegend), Biotinylated CD71 (R17217; Biolegend), PD1‐PerCP/Cy5.5 (29 F.1A12; Biolegend), NKp46‐PE‐Cy7 (29A1.4; eBioscience), Tim3‐PE/Cy7 (RMT3‐23; Biolegend), Gr1‐APC (RB6‐8C5; BD), Ly6c‐BV510 (HK1.4; Biolegend), Lsel‐AF700 (MEL‐14; Biolegend), Sca‐1‐PerCP/Cy5.5 (D7; Invitrogen), Ter119‐BV510 (TER‐119; Biolegend), Streptavidin‐PE/Cy7 (Biolegend).

Techniques: Expressing, Activity Assay, Activation Assay, Marker

Journal: Cell Reports Medicine

Article Title: Probiotics and their metabolite spermidine enhance IFN-γ + CD4 + T cell immunity to inhibit hepatitis B virus

doi: 10.1016/j.xcrm.2024.101822

Figure Lengend Snippet:

Article Snippet: Anti-mouse NKp46 (29A1.4) PE-CY7 , eBioscience , Cat# 25-3351-82; RRID: AB_2573442.

Techniques: Staining, Virus, Capsules, Recombinant, Control, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, AST Assay, Northern Blot, Sequencing, Transgenic Assay, Software

Journal: Heliyon

Article Title: A comprehensive analysis of the immune system in healthy Vietnamese people

doi: 10.1016/j.heliyon.2024.e30647

Figure Lengend Snippet: T and NK Cell Activating Receptors in Healthy Vietnamese Adults. This table presents the mean ± standard deviation (SD), Min-Max % and 95 % CI data for a panel of activating receptors expressed by T and NK cells in 40 healthy Vietnamese individuals.

Article Snippet: , , , Hu CD335 (NKP46) PE-Cy7 9E2/NKP46 , BD Biosciences , 5 μl.

Techniques: Standard Deviation

Journal: Heliyon

Article Title: A comprehensive analysis of the immune system in healthy Vietnamese people

doi: 10.1016/j.heliyon.2024.e30647

Figure Lengend Snippet: Marker antigens, reagents with coupled fluorochromes, distributors, and Ab volume used to stain whole blood cells were listed.

Article Snippet: , , , Hu CD335 (NKP46) PE-Cy7 9E2/NKP46 , BD Biosciences , 5 μl.

Techniques: Marker, Staining